Authors
1
Kirkuk university-college of agriculture-department of horticulutre
2
Dept. of Horticulture and Landscaping Design\ College of Agriculture\ University of Kirkuk\Kirkuk\ IRAQ
3
Dep. Horticulture, College of Agriculture, University of Kirkuk, Kirkuk, IRAQ
,
Document Type : Research Paper
Abstract
This experiment was carried out in the Plant Cells and Tissue Culture Laboratory of Horticulture and Landscaping Design Department / College of Agriculture / University of Kirkuk - IRAQ, to study the effect of auxin’s in different concentrations and MS media (full and ½) salt strength on rooting response of Petunia hybrida. The regenerated shoots explants from the multiplication stage were transferred to the rooting stage cultured on MS media ( full and ½ ) salts strength supplemented with different concentrations of IBA at ( 0.0, 0.25, 0.50, 0.75, and 1.0 ) mg.L-1, and NAA at ( 0.0, 0.25, 0.50, 0.75, and 1.0 ) mg.L-1. After 6 weeks of transforming, data showed that, the response percentage was ( 100% ) for MS media ( full and ½ ) salt strength supplemented with different concentrations of IBA and NAA beside control treatment. The best number of roots ( 29.70 root.part-1 ) and the length of the longest root ( 7.44 cm ) were recorded by MS ½ strength supplemented with IBA (1.0 mg.L-1) compared with other concentrations, and it was the highest results compared with MS full strength supplemented with different concentration of IBA. Moreover, results obtained that MS ½ strength supplemented with NAA ( 1.0 mg.L-1 ) recorded the best number of roots ( 18.70 root.part-1 ) and the length of the longest root ( 2.97 cm ) compared with the other concentrations, while it was the best result compared with MS full strength supplemented with different concentrations of NAA.
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