Kirkuk University Journal for Agricultural Sciences (KUJAS)

Influence of Auxins and different growths media strength on rooting of Petunia hybrida L. In vitro Propagation

https://doi.org/10.58928/ku24.15315
Volume 15, Issue 3
Summer 2024
Page 126-132
Kirkuk University Journal for Agricultural Sciences (KUJAS)

Document Type : Research Paper

Authors

Taban Falah Kamal 1
Kefaia Alsaad 2
Ali mohammed Noori 3

1 Kirkuk university-college of agriculture-department of horticulutre

2 Dept. of Horticulture and Landscaping Design\ College of Agriculture\ University of Kirkuk\Kirkuk\ IRAQ

3 Dep. Horticulture, College of Agriculture, University of Kirkuk, Kirkuk, IRAQ

Abstract
This experiment was carried out in the Plant Cells and Tissue Culture Laboratory of Horticulture and Landscaping Design Department / College of Agriculture / University of Kirkuk - IRAQ, to study the effect of auxin’s in different concentrations and MS media (full and ½) salt strength on rooting response of Petunia hybrida. The regenerated shoots explants from the multiplication stage were transferred to the rooting stage cultured on MS media ( full and ½ ) salts strength supplemented with different concentrations of IBA at ( 0.0, 0.25, 0.50, 0.75, and 1.0 ) mg.L-1, and NAA at ( 0.0, 0.25, 0.50, 0.75, and 1.0 ) mg.L-1. After 6 weeks of transforming, data showed that, the response percentage was ( 100% ) for MS media ( full and ½ ) salt strength supplemented with different concentrations of IBA and NAA beside control treatment. The best number of roots ( 29.70 root.part-1 ) and the length of the longest root ( 7.44 cm ) were recorded by MS ½ strength supplemented with IBA (1.0 mg.L-1) compared with other concentrations, and it was the highest results compared with MS full strength supplemented with different concentration of IBA. Moreover, results obtained that MS ½ strength supplemented with NAA ( 1.0 mg.L-1 ) recorded the best number of roots ( 18.70 root.part-1 ) and the length of the longest root ( 2.97 cm ) compared with the other concentrations, while it was the best result compared with MS full strength supplemented with different concentrations of NAA.

Keywords

Auxin
Petunia hybrida
MS
In Vitro
IBA
NAA
Subjects

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