Kirkuk University Journal for Agricultural Sciences (KUJAS)

Morphological description and Molecular identification of species Phyllotreta striolata (Coleoptera: Chrysomelidae: Alticinae) in Erbil, Iraq

https://doi.org/10.58928/ku25.16427
Volume 16, Issue 4 - Issue Serial Number 4
Autumn 2025
Page 249-255
Kirkuk University Journal for Agricultural Sciences (KUJAS)

Document Type : Research Paper

Authors

Muthanna jamal jawad 1
Hozan Qader hamamurad 1
azhin mohamed pirbal 2
banaz salah abdullah 3
nabeel abdulqader mawlood 2

1 department of plant protection , College of Agricultural Engineering Sciences, Salahaddin University– Erbil, Iraq.

2 Plant Protection Department, College of Agricultural Engineering. Science, Salahaddin. University– Erbil., Iraq.

3 Biology Department, College of Education, Salahaddin. University– Erbil., Iraq.

Abstract
ABSTRACT
The detail description of the striped flea beetle Phyllotreta striolata (Coleoptera: Chrysomelidae : Alticinae ) and a Molecules have been identified in the Erbil city. Samples were gathered by using an Arial insect net to collect from the Capparis spinosa plant during period from 1 / October / 2024 till 22 / November / 2024 . The important characters of species are , mandibles apical parts consist of four tentacles , maxillary palp consists of four segments . The Antennae pale brown- brown consist of 11th antennomers , 1st-5th antennomer pale brown , 5th antennomer 2 times as long as the 6th antennomer , swollen , 11th antennomer oval elongated . The elytra dark brown , middle part pale yellow. Speculum gaster is Y-shaped. Aedeagus is pale brown , tubular-shaped . Pictures of the key parts were included . They show where each sample was found , what plants they were on , and the date they were collected .
The molecular identification discover a 550 bp bands of mitochondrial gene COI , which was amplified with PCR from the Phyllotreta striolata for constructing and distinguish a phylogenetic tree . The COI gene sequences of sample species were aliment insides NCBI GenBank using BLAST programs and used to compare the sequenced nucleotides others Phyllotreta species sequence . The outcome demonstration that obtained sequences was P. striolata identified species based on the mitochondria COI gene . The sequence COI of P. striolata was submitted to GenBank with accession PV123233 .

Keywords

Keyword: Morphological
Molecular
Phyllotreta
striolata
Chrysomelidae
Subjects
  • Konstantinov, A. S. & Vandenberg, N. J. (1996). Handbook of Palearctic flea beetles (Coleoptera: Chrysomelidae: Alticinae). Contributions on Entomology, International, 1: 236-439.
  • Konstantinov, A. S. & Vandenberg, N. J. (2015). Guide to Palearctic Flea Beetle Genera (Coleoptera: Chrysomelidae: Alticinae). http: // www . barc. Usda . gov /coleoptera/ fleabeetles/fleas.htm [accessed 20 February 2015].
  • Wu, Z., Bin, S., He, H., Wang, Z., Li, M. and Lin, J., (2016). Differential expression analysis of chemoreception genes in the striped flea beetle Phyllotreta striolata using a transcriptomic approach. PLoS One, 11(4), p.e0153067.
  • Özdikmen, H., Coral Şahin, D. and Bal, N., (2017). Phyllotreta Chevrolat in Turkey with a new record (Chrysomelidae: Galerucinae: Alticini). Munis Entomology & Zoology, 12(1).
  • Warchałowski, A. (2003). Chrysomelidae: the leaf beetles of Europe and the mediterranean Area. Natura Optima Dux Foundation, Warszawa, 600 pp.
  • Warchałowski, A. (2010). The Palaearctic Chrysomelidae: Identification keys, Vol 1 & 2. Natura Optima Dux Foundation, Warszawa, 1212 pp.
  • HEBERT, P.D., CYWINSKA, A., BALL, S.L. AND DEWAARD, J.R., (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270(1512), pp.313-321.
  • SÁNCHEZ-GEA, J.-F., GALIAN, J. & SERRANO, J. (2004). Phylogeny of Iberian Zabrus (Coleoptera: Carabidae: Zabrini) based on mitochondrial DNA sequence. European Journal of Entomology, 101, 503-511.
  • Lee, C.F., Chang, H.Y., Wang, C.L. and Chen, W.S., (2011). A Review of Phyllotreta Chevrolat in Taiwan (Coleoptera: Chrysomelidae: Galerucinae: Alticini) . Zoological Studies, 50(4), pp.525-533.
  • MAWLOOD, N.A., HAMAD, M.I. and ABDULLAH,Y. M. (2016). A new record of glaphyrid scarab beetles, Eulasia vitatta (Fabricius, 1775) (Coleoptera, Glaphyridae) from Erbil Kurdistan region-Iraq. Zanco Journal of Pure and Applied Sciences, 28(3), pp.1-4.
  • Hammamurad, H.Q.; Mawlood, N.A. and Omar, Z.Z. (2024). Morphological study and Molecular identification of Maiestas knighti (Webb and Viraktamath, 2009) (Hemiptera: Cicadellidae) in Erbil Province, Kurdistan Region –Iraq. Kirkuk University Journal for Agricultural Sciences, Vol. 15, No. 1, 2024 (286-294)
  • Stephens, R., Horton, R., Humphray, S., Rowen, L., Trowsdale, J., and Beck, S. (1999). Gene organization, sequence variation and isochore structure at the centromeric boundary of the human MHC. J. Mol. Biol., 291, 789–799.
  • LIU, X. F., YANG, C. H., HAN, H. L., WARD, R. D. & ZHANG, A. B. (2014). Identifying species of moths (Lepidoptera) from Baihua Mountain, Beijing, China, using DNA barcodes. Ecol Evol, 4, 2472-87. https://pmc.ncbi.nlm.nih.gov/articles/PMC4203292/pdf/ece30004-2472.pdf
  • JALALI, S., OJHA, R. & VENKATESAN, T. (2015). DNA barcoding for identification of agriculturally important insects. New horizons in insect science: Towards sustainable pest management. Springer. https://www.researchgate.net/publication/276849159
  • Coral Şahin D, Magoga G, Özdikmen H, Montagna M. DNA Barcoding as useful tool to identify crop pest flea beetles of Turkey. (2019). J Appl Entomol. 143:105–117. https://doi.org/10.1111/jen.12566.
  • MA, T.T., LIN, F., ZHAO, N., RUAN, Y.Y., XIE,S.Y.  ZOU, H.D. CHEN, J.Y., FANG, B.P. and   HUANG, L.F. (2022). Identification and mitochondrial genome analysis of the sweetpotato flea beetle, Chaetocnema confifinis (Coleoptera: Chrysomelidae), an invasive pest in the Chinese mainland. Acta Entomologica Sinica, 65 (10): 1354 – 1366.

 

 

Home

Submit Manuscript

Editorial Board

Aims and Scope

Animal and Ethical Approval

Publication Ethics

Guide for Authors

Guide for Reviewer

Indexing and Abstracting

DOI Use

Generative AI (GenAI) Policy

Data Availability and Reproducibility Policy

Peer Review Process

Copyright Policies

Self Archiving Policy

The Time Period of Reviewing Process

Privacy Statement

Plagiarism Policy

Article Processing Charges (APC)

Reviewers

Contact Us