Authors
1
Faculty of agriculture Saba Basha Alexandria University
2
sugar Crops Research Institute; Agricultural Research Center (ARC); Ministry of Agriculture, Egypt
3
Department of Nucleic Acids Research, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technology Applications
4
Sugar Crops Research Institute; Agricultural Research Center (ARC); Ministry of Agriculture, Egypt
5
Agricultural Botany Department, Faculty of Agriculture (Saba-Basha), Alexandria University, Egypt
,
Document Type : Research Paper
Abstract
Stevia rebaudiana is a small perennial shrub of Astraceae family. Stevia rebaudiana is a small perennial shrub of Astraceae family. The leaves of this plant contain different diterpenc glycosides. The leaves of this plant contain different diterpenc glycosides. The herb produces a high potency and zero calorie sweeteners in its leaf tissue (stevioside and rebaudioside A). Stevia plant produces a low-calorie sweetener in its leaves, which is being a calorie free sweetener, considered a helpful food additive for people with hypertension, diabetes, and obesity. The highly potent diterpene glycosides (stevioside and rebaudioside A), sweeteners are 300 times sweeter than sucrose. The aim of the present study is to investigate the effect of micronutrients applications on diterpene glycosides (stevioside and rebaudioside A) in three stevia varieties using HPLC analysis technique and investigating the expression levels of UGT76G1 gene using gene-specific primers. In this context stevia sweeteners (Stevioside and Rebaudioside A) leaves contents were extracted and determined. The results indicated that different treatments resulted in variable steviol glycoside contents. In addition, gene expression level of UGT76G1 was affected with the micronutrient applications, as an increase in the transcription abundance of the gene was observed. In conclusion, micronutrients were found to enhance stevia glycosides accumulation in the leaf tissues, especially in Egy1 and China1 varieties. In addition, the application of the three micronutrients (Zn, B and Mo) depicted the most favorable effects on stevia leaves. The results also showed that variety China1 glycosides were increased in treatments T6, T7 and T8. Finally, in variety Sponti, the highest value of stevia glycosides was obtained with T5 and T8 treatments.
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