This study was carried out to determine the physiological effect of each of biofertilizer EM1, organic fertilizer Biosol-N and metal nitrogen fertilizer on growth and yield and its’ components of upland cotton (Gossypium hirsutum L.) Varity Lachata. The study included a field experiment at two locations through 2010 season. The first location at The Experimental Station of Field Crops Department, collage of Agriculture and Forestry - University of Mosul, while the second at Al- Hawija (48 km) away southwest of Kirkuk. Three factors were tested using factorialrandomized complete block design. with three replications, the factors included three levels of Biofertilizer (EM1) (0 , 1 and 2 ml / liter) and three levels of Organic fertilizer (Biosol-N) (0 , 250 and 500 kg / ha) and two levels of nitrogen fertilizer (0 and 200 kg N / ha) respectively. The results indicates that Biofertilizer EM1 caused significant increasing in all growth properties, yield and its’ components at Mosul and Al-Hawija locations. Organic fertilizer “Biosol-N” showed significant increase in all growth properties, yield and its’components at Mosul and Al-Hawija locations. The Nitrogen fertilization had a significant effect on dry weight of leaves and net assimilation rate at Mosul and Al-Hawija locations, plant high and crop growth rate at Al-Hawija location.The effect of nitrogen fertilization was significant for all yield and it’s components at Mosul and Al-Hawija locations except No. of bolls, ginning outturn at Mosul location