A aim of this study was to investigate effect of the interaction between Biofertilizer EM1 (0 and 1.5 ml.l-1), phosphorus fertilizer (0 , 40 , 80 and 120 kg P2O5.ha-1) on vegetative and fruitful growth to two soybean varieties (Lee-74 and Senaia-2). The field experiment was conducted in two locations, the first in rea- sreches station of department of field crops/College of Agriculture and Forestry / Mosul University in Mosul City, while the second was in Tuzkhurmatu City by using RCBD design with three replications. The results showed that interaction (1.5 ml.l-1 EM1× 80 kg P2O5.ha-1) in Mosul location and (1.5 ml.l-1 EM1× 40 kg P2O5.ha-1) in Tuzkhurmatu location gave highest rate in most of traits were studied compared with non fertilizer treatment. Interaction between biofertilizer EM1 and varieties were Significantly for traits no. of fruitful clusters/branches and no. of fruitful clusters/plant in mosul location only. There were a Significant interaction between phosphorus fertilizer and varieties for some traits in both locations. The interaction among (1.5 ml.l-1 EM1 × 80 kg P2O5.ha-1 ×Senaia-2 variety) in Mosul location gave highest rate in plant height at full flowering, no.of fruitful clusters / branches, no. of fruitful clusters/plant, no.of pod/branches and pod lenghth, while the interaction among (1.5 ml.l-1 EM1 × 40 kg P2O5.ha-1 ×Senaia-2 variety) in Tuzkhurmatu location gave highest rate in no. of node/main stem and pod lenghth.