Kirkuk University Journal for Agricultural Sciences (KUJAS)

In Vitro Propagation of Pyracantha coccinea as Affected by Growth Regulators and Different Carbon Sources

https://doi.org/10.58928/ku22.13421
Volume 13, Issue 4
Autumn 2022
Page 244-252
Kirkuk University Journal for Agricultural Sciences (KUJAS)

Document Type : Research Paper

Authors

Waad S. Faizy 1
Rafail S. Toma 2
Yousif S. Tamer 3
Wisam Khazaal 4

1 Dep. of Plant Production Techniques, Agricultural Technical College, Northern Technical University, Iraq

2 Dep. of Horticulture, College of Agricultural Engineering Sciences, University of Duhok, Kurdistan Region-Iraq

3 Ministry of Education, Kurdistan Region-Iraq

4 Department of Horticulture & Landscape, College of Agriculture & Forestry, Mosul University, Iraq

Abstract
The aim of this study was to investigate the effect of different types and concentration of plant
growth regulators (Cytokinins: Kinetin and Benzyl adenine (BA) at 0, 0.5, 1, 1.5, 2 and 2.5 mg.l-
1) on shoot proliferation of Pyracantha coccinea and auxin (indole-3- butyric acid) (IBA at 0, 0.5
and 1 mg.l-1) and different carbon sources (Sucrose and Glucose) with ½ and full MS salt strength
in root formation. The results obtained from this study indicate that the greatest number shoots and
leaves per explant were acquired when BA was 2.5 mg/l-1, which gave 8 shoots and 54.33 leaves
respectively. In the rooting stage, the greatest number of roots per explant was obtained when half
MS salt strength was used with Sucrose and 1mg.l-l IBA, which gave 11.33 roots/explant. Up to
83% of shoots were rooted. plantlets of P. coccinea with roots were successfully acclimatized with
a survival rate of 80% in sand, 95% in peatmoss and 85% in sand + peatmoss. This procedure can
be adopted for P. coccinea in vitro propagati

Keywords

Pyracantha coccinea
PGRs
carbon source
Subjects

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