Document Type : Research Paper
Abstract
The aim of this study was to investigate the effect of different types and concentration of plant
growth regulators (Cytokinins: Kinetin and Benzyl adenine (BA) at 0, 0.5, 1, 1.5, 2 and 2.5 mg.l-
1) on shoot proliferation of Pyracantha coccinea and auxin (indole-3- butyric acid) (IBA at 0, 0.5
and 1 mg.l-1) and different carbon sources (Sucrose and Glucose) with ½ and full MS salt strength
in root formation. The results obtained from this study indicate that the greatest number shoots and
leaves per explant were acquired when BA was 2.5 mg/l-1, which gave 8 shoots and 54.33 leaves
respectively. In the rooting stage, the greatest number of roots per explant was obtained when half
MS salt strength was used with Sucrose and 1mg.l-l IBA, which gave 11.33 roots/explant. Up to
83% of shoots were rooted. plantlets of P. coccinea with roots were successfully acclimatized with
a survival rate of 80% in sand, 95% in peatmoss and 85% in sand + peatmoss. This procedure can
be adopted for P. coccinea in vitro propagati
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