Abstract
The results of serological diagnosis using TAS-ELISA test showed presence of cucumber mosaic virus in samples brought from the cucumber fields. Using systemic inducing resistance agents Azospirillum brasilense, Trichoderma harzianum T-22 , and their mixture improved pepper vegetative parameters. Seedlings treatment with A. brasilense improved in shoot, root wet and dry weight 14.32 , 4.11, 16.48 and 5.43 gm respectively, while it were 9.48 ,2.75,14.89 and 3.72 gm, respectively, in control treatment. The amount of chlorophyll was 2.065 mg. g-1 wet weight, while it was 1.183( mg. g-1 wet weight-1) in control treatment. leaf area was 133.17cm2, while it was 95.51 cm2 control treatment. Pepper seedlings treatment with A. brasilense caused raising in peroxidase enzyme activity, polyphenol oxidase enzyme activity and total phenols after thirty days of treatment with the virus.Pepper seedlings treatment raising in peroxidase enzyme activity with 285.85(unit.min-1.g wet weight-1), while it was 107.42(unit.min-1.g wet weight-1) in control treatment. In polyphenol oxidase enzyme activity it was 114.41(unit.min-1.g wet weight-1) 266.81
respectively (unit.min-1.g wet weight-1) in control treatment .The highest amount of total phenols was recorded in pepper seedling treated with mixture of T. harzianum T-22 and A. brasilense with 4.6.(mg-1.g ww-1) while it was 1.15 (unit.min-1.g wet weight-1) in control treatment
Main Subjects
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