The result of serological diagnosis using TAS-ELISA test showed presence of cucumber mosaic virus in samples of squash plant from the fields in Mosul city .pepper seeds treated with Trichoderma harizanum T-22 and Azospirillum brasilense caused an increase in peroxidase enzyme, polyphenol oxidase enzymes activity and phenol content in plants infected with cucumber mosaicvirus .Where the highest values of increasing activity were recorded. peroxidase on the thirtieth day after infection with the virus in T. harizanum T-22 treatment with 474.21 (unit. min.g wet weight-1) while the activity of the enzyme was 161.4 (unit. .min.g wet weight-1) in control treatment . mixture of A.brasilense and T.harizanum T-22 treatment showed the highest level of polyphenol oxidase activity with 345.37 (unit. min.g wet weight-1) after thirty days of treatment. In control treatment plants, the enzyme activity was 127.1 (unit. min.g wet weight-1) .
The highest amount of total phenols was recorded in mixture of A.brasilense and T.harizanum T-22 treatment with 1.35(mg.g wet weight-1), while the lowest amount was 0.85 (mg.g wet weight-1) in control treatment