The study was conducted during the growing season 2012-2013 to determine the effect of row spacing in growth. yield and its components of Barley. The experiment carried out in field in Nineveh province in Gleolkhan location which is moderate rainfall area. it is about 15 miles far from the Mosul city. The field experiment included two factors: the first was two cultivars of barley (two rows and six-rows) and the second was row spacing (conventional planting after tillage with row spacing 15 cm., zero tillage planting system with two row spacing 15 cm and 30 cm. and finally zero tillage cross planting 30 cm x 30 cm). Split plot design were applied in randomize complete block design with three replicates. The effects of the above two factors and its interactions on growth. yield and its components for two cultivars of barley was studied and the results were as follows: Adroh cultivar achieved highest significant value in the traits (number of tillers / m 2 and number of spikes / m 2). Mutaa cultivar achieved a higher significant values in the traits (number of grains per spike and the percentage of protein in grain) and there was no significant difference between the two cultivars weed dry weight / m2. flag leaf area cm2, plant height cm, biological weight, weight of grain yield, straw yield, 1000-grain weight and harvest index. Cross planting 30 x30 cm achieved highest significant values in (flag leaf area. the number of tillers/m2, number of spikes/m2, biological weight, weight of grain and straw yield. the difference was not significant with conventional tillage, whereas there was no significant difference between all second factor levels in the traits of plant height, number of grains in spike. 1000-grain weight and harvest index. The interaction between Mutaa and Adroh cultivars with zero tillage cross planting 30 x30 cm method gave highest grain yield gm/m2.