The daffodil flower is one of the commercial ornamental plants in the world today for cut flowers and garden plants. The aim of the study is to determine the influence of arbuscular mycorrhiza fungi (Glomus intraradices) with phosphorus on growth of daffodil plants in calcareous soil. The application of arbuscular mycorrhiza fungi (AMF) with different levels of phosphorus (0, 40, 80, 120, and 160 kg ha-1) significantly affected daffodil characteristics. The treated plants with AMF and 160 kg ha-1 phosphorus (P) gave the superior spike length (49.00 cm), spike diameters (9.84 mm), flower diameter (57.76 mm), leaf number (8.33), leaf length (51.33 cm), chlorophyll intensity (136.78 SPAD), leaf dry matter (16.49 %), root number (67.33), root length (35.00 cm), nitrogen (17.90 g kg-1), and potassium (19.23 g kg-1). On the other hand, the plants were treated with AMF and 80 kg ha-1 P resulted in the maximum root colonization (72 %), phosphorus content (13.23 g kg-1), iron (345.67 mg kg-1), and zinc (86.33 mg kg-1) contents. In contrast, the minimum results in this study were found in non-inoculant plants with untreated phosphorus. Based on the results of this study, application of AMF along with 160 kg ha-1 P doses was recommended for improving flower and growth characteristics of daffodil.