Abstract
This study focused on the preventive effects of powdered Arugula (Eruca sativa) seeds on some physiological and biochemical parameters in male local rabbits that were stressed by adding 1% hydrogen peroxide(H2O2). The purpose of this experiment was to investigate the protective function of E. sativa seeds against oxidative stress caused by 1% H2O2. 32 male rabbits from the area were divided randomly into four groups (8rabbits/treatment). The experiment diets were: T1=control group, T2= adding 3g/kg diets of E. sativa seeds powder, T3= adding 1% of H2O2 in water, T4= adding 3g/kg diets of E. sativa seeds powder and 1% of H2O2 in water. At 42 days into the experiment, blood samples were also taken, and the following parameters were measured: HG, MCV,MCH, and MCHC in blood (RBCs), Total number of WBCs, lymphocyte,neutrophils,MID percentage. Immunoglobulin’s (IgG and IgM) levels, Urea, Creatinine and testosterone.According to our findings, administering 1%H2O2 significantly (P≤0.05) reduced the amount of total RBCs, HG,MCV,MCH,MCHC,IgG and IgM.Significantly increased the levels of urea and creatinine compared to other treatments. The results also showed that adding 3g/Kg diet of rocket (E.sativa) seeds resulted in a significant (P≤0.05) decrease in urea and creatinine and an increase in the mean values of RBCs,HG,MCV,MCH, and MCHC,as well as IgG and IgM. These results suggested that administering 1% hydrogen peroxide/water causes oxidative stress, and lead to increase urea and creatinine, and a decrease in MCV,MCH and MCHC. Adding E. sativa caused decrease levels of IgG and IgM and minimize the toxic effect of hydrogen peroxide.
Main Subjects