Document Type : Research Paper
Authors
1
Department of Nursing, Technical College of Health and Medical technology, Sulaimani polytechnic university, Sulaymaniyah, Kurdistan Region, Iraq
2
Department of Medical Laboratory Science, Technical College of Applied Science, Sulaimani Polytechnic University, Sulaymaniyah, Kurdistan Region, Iraq.
3
Department of Horticulture, College of Agricultural Engineering Sciences, University of Raparin, Rania, Kurdistan Region, Iraq.
Abstract
This study focused on the preventive effects of powdered Arugula (Eruca sativa) seeds on some physiological and biochemical parameters in male local rabbits that were stressed by adding 1% hydrogen peroxide(H2O2). The purpose of this experiment was to investigate the protective function of E. sativa seeds against oxidative stress caused by 1% H2O2. 32 male rabbits from the area were divided randomly into four groups (8rabbits/treatment). The experiment diets were: T1=control group, T2= adding 3g/kg diets of E. sativa seeds powder, T3= adding 1% of H2O2 in water, T4= adding 3g/kg diets of E. sativa seeds powder and 1% of H2O2 in water. At 42 days into the experiment, blood samples were also taken, and the following parameters were measured: HG, MCV,MCH, and MCHC in blood (RBCs), Total number of WBCs, lymphocyte,neutrophils,MID percentage. Immunoglobulin’s (IgG and IgM) levels, Urea, Creatinine and testosterone.According to our findings, administering 1%H2O2 significantly (P≤0.05) reduced the amount of total RBCs, HG,MCV,MCH,MCHC,IgG and IgM.Significantly increased the levels of urea and creatinine compared to other treatments. The results also showed that adding 3g/Kg diet of rocket (E.sativa) seeds resulted in a significant (P≤0.05) decrease in urea and creatinine and an increase in the mean values of RBCs,HG,MCV,MCH, and MCHC,as well as IgG and IgM. These results suggested that administering 1% hydrogen peroxide/water causes oxidative stress, and lead to increase urea and creatinine, and a decrease in MCV,MCH and MCHC. Adding E. sativa caused decrease levels of IgG and IgM and minimize the toxic effect of hydrogen peroxide.
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