Phytochemical Diversity in Seven Grapevine Varieties Using GC-MS

Document Type : Research Paper

Authors

1 University of Anbar

2 University of Anbar/Center of Desert studies

3 Department of Horticulture, College of Agricultural Engineering Sciences, University of Duhok, Kurdistan Region, Iraq

4 Center of Desert Studies, University of Anbar, Anbar, IRAQ

Abstract
The aim of the current study was to extract the active compounds of seven grape varieties cultivated in Duhok Governorate, Kurdistan Region of Iraq, for the 2023 season. The varieties included (Hejazi, Tre rash, Eayn althawr, Superior seedless, Moon drop, Crimson, Samarkandi). The results indicated that the leaves of grape varieties contain many active compounds, and the number and concentrations of these active compounds vary among the studied varieties. The number of active compounds according to the sequence was Hejazi 61 with highest compound of Methyl linolenate (6.71%), Superior seedless 36, Moon drop 33, Tre rash and Crimson 22, Eayn althawr 14, and Samarkandi 13 with 22.50 of α-Tocospiro-B . The compound Methyl palmitate appeared at its highest levels in the varieties (Tre rash, Eayn althawr, Superior seedless, Moon drop, Crimson), with values of (16.02%, 26.53%, 21.39%, 18.29%, 32.16%), respectively. GC-MS analysis confirmed that there is a biochemical diversity in the given grapevine varieties. Therefore, these varieties could distinguish via the highest compound appeared in GC-MS analysis. The aim of the current study was to extract the active compounds of seven grape varieties cultivated in Duhok Governorate, Kurdistan Region of Iraq, for the 2023 season. The varieties included (Hejazi, Tre rash, Eayn althawr, Superior seedless, Moon drop, Crimson, Samarkandi). The results indicated that the leaves of grape varieties contain many active compounds, and the number and concentrations of these active compounds vary among the studied varieties. The number of active compounds according to the sequence was Hejazi 61 with highest compound of Methyl linolenate (6.71%), Superior seedless 36, Moon drop 33, Tre rash and Crimson 22, Eayn althawr 14, and Samarkandi 13 with 22.50 of α-Tocospiro-B .

Keywords

Subjects
  • Insanu, M., Karimah, H., Pramastya, H. and Fidrianny, I. (2021). Phytochemical compounds and pharmacological activities of Vitis vinifera: an updated review. Biointerface Res. Appl. Chem., 11(5), 13829-13849.
  • Saeedi, I.H.M. (2000). Production of grapes. Ed books for printing and publishing. University of Al Mosul. Iraq. 223p.
  • Abdul-Qader, M. (2006). Effect of training systems, canopy management and dates on the yield and quality of grape-vines cu "Taifi" ( Vitis vinifera L.).under nonirrigated conditions . M.S.C. Thesis. Dohuke university. Iraq. 176p.
  • Hassan, J.A. and Salman, M.A. (1989). Production of grapes. House of Wisdom, University of Baghdad, Ministry of Higher Education and Scientific Research, Iraq. 168p.
  • Gamal El Din, F.A. (2010). Encyclopedia of medicinal plants. Second Edition. Origin of knowledge. Alexandria. The Egyptian Arabic Republic. 134p.
  • Valli Kanagarla, N.S.S.A., Kuppast, I.J., Veerashekar, T. and Reddy, C.L.A. (2013). review on benefits and uses of Vitis vinifera (Grape). RRBS., 7(5), 175-180.
  • Marjan, N. and Hosseinzadeh, H. (2016). Review of the Pharmacological Effects of Vitis vinifera (Grape) and its Bioactive Constituents. Phytother. Res., 30, 1392–1403.
  • Jayshree, A. and Ogale, S.A. (2023). Review on Benefits and Uses of Vitis Vinifera (Grapes). International Journal of Research Publication and Reviews., 4(3),.2321-2335.
  • Thiago, A., De Melo, G., Júnior, W.S.F. and De Albuquerque, U.P. (2016). Medicinal Plants. in Introduction to Ethnobiology., 143-149.
  • Azmir, , Zaidul, I.S.M., Rahman, M.M., Sharif, K., Mohamed, M.A., Sahena, F., Jahurul, M.H.A., Ghafoor, K.N.A., Norulaini, N. and Omar, A.K.M. (2013). “Techniques for Extraction of Bioactive Compounds from Plant Materials A Review.” Journal of Food Engineering., 117, 426-436.
  • Jayaprakasha, G.K., Selvi, T. and Sakariah, K.K. (2003). Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts, Food Res.Int., 36, 117-122.
  • Bilyk, A. Cooper, P.L. and Sapers, G.M. (1984). Varietal differences in distribution of Quercetin and kaempferol in onion (Allium cepa) tissue. J. Agric. Food Chem., 32, 274-276,
  • Muhammad, I., Rauf, A., Imran, A., Nadeem, M., Ahmad, M., Atif, Z.M., Wais, A.M., Sami, M., Imran, M., Fatima, Z. and Waqar, B. (2017). Health Benefits of Grapes Polyphenols. Journal of Environmental and Agricultural Sciences., 10, 40-51.
  • Alazzam, S.A., Mohammed, M. Sh. and Ali, F.A. (2020). Phytochemical analysis, antioxidant and cytotoxic potential of Rumex vesicarius extracts. medico-legal update., 20, 449-454.
  • Alazzam, S.A., Sharqi, M.M. and Almehemdi, A.F. (2021). Allelochemicals analysis of Rumex vesicarius and Zygophyllum coccineum L., and their effect on seed germination and seedling growth of wheat, Triticum aestivum L. IOP Conf. Series: Earth and Environmental Science, 761: 012077.
  • Mohammed, A.M., Zeyad, M.A. and Almehemdi, F.A. (2020). Active compounds analysis in five Roselle varieties using GC/MS. International Journal of Agricultural and Statistical Sciences., 16,.12251233.
  • Alfalahi, A O., Alrawi, M.S., Theer, R.M., Dawood, K.F., Charfi, S. and Almehemdi, A.F. (2024). Phytochemical analysis and antifungal potential of two Launaea mucronata (Forssk.) Muschl and Launaea nudicaulis Hook.fil. wildly growing in Anbar province, Iraq. J. Ethnopharmacol., 10, 116965.
  • Jamea, F.F., Al-Kaisey, M.T. and Al-Maliky, Z.S. (2010). Determination of phenol components in rachis and leaves of some grape varieties (Vitis vinifera). Ibn Al-Haitham J. for Pure and Appl. Sci., .23(1), 1-11.
  • Khan, Y., Khan, S.M., UlHaq, I., Farzana, F., Abdullah, A., Abbasi, A.M., Alamri, S., Hashem, M., Sakhi, S., Asif, M. and Shah, H. (2021). Antioxidant potential in the leaves of grape varieties (Vitis vinifera) grown in different soil compositions. Arabian Journal of Chemistry., 14(11), 1-10.
  • Nzekoue, F.K., Nguefang, M.L.K., Alessandroni, L., Mustafa, A.M., Vittori, S. and Caprioli, G. (2022).  Grapevine leaves (Vitis vinifera): Chemical characterization of bioactive compounds and antioxidant activity during leave development., 50(102120), 13-25.
  • Singh, J., Rasane, P., Kaur, R., Kaur, H., Garg, R., Kaur, S., Ercisli, S., Choudhary, R., Skrovankova, S. and Mlcek, J. (2023). Valorization of grape (Vitis vinifera) leaves for bioactive compounds: novel green extraction technologies and foodpharma applications. Front. Chem. 11(1290619): 1-22.
  • Alrawi, M.Sh., Aldubaisi, I.A. and Almehemdi, A.F. (2023). Chemotaxonomy of Twelve Mimosoideae Species in Iraq. IOP Conf. Ser.: Earth Environ., Sci. 1252, 012085.
  • Alfalahi, A.O., Hussein, Z.T., Khalofah, A., Sadder, M.T., Qasem, J.R., Al-Khayri, J.M., Jain S.M. and Almehemdi, A.F. (2022). Epigenetic variation as a new plant breeding tool: A review. Journal of King Saud University-Science., 34, 102302.
  • Seigler, D.S. (2012). Plant Secondary Metabolism. Springer Science & Business Media.
  • Calixto, J.B. (2000). Efficacy, Safety, Quality Control، Marketing and Regulatory Guidelines for Herbal Medicines (Phytotherapeutic Agents). Brazilian Journal of Medical and Biological Research., 33(2),179–189.
  • Srivastava, K. (2018). “Significance of Medicinal Plants in Human Life.” Pp. 1-24 in Synthesis of Medicinal Agents from Plants. Elsevier.
  • Awuchi, C.G. (2019). Medicinal plants: the medical, food, and nutritional biochemistry and uses. International Journal of Advanced Academic Research., 5(11), 220-241