A study was conducted in village of Sheikh Mohammed (Badoosh Dam) for summer season 2014, The experiment included three factors: four levels of nitrogen fertilization (0, 120, 240 and 360 kg N/ha), three plant spaces (20, 30 and 40 cm), and third three varieties of upland cotton (Lachata, Coker 310 and Montana). For study lessening boll shedding %, the experiment was conducted by using Randomized Complete Block Design with three replicates, The results illustrated that level 240 kg N/ha surpass in the characters of boll shedding (2.49%), number of total bolls (33.45 boll/plant), seed index (9.98 g), plant yield (93.98 g) and earliness (80.74 g). The results revealed that plant space 30cm in the characters of number of nodes to first fruiting (3.14 node), boll shedding (2.33%), seed index (9.85 g) and earliness (78.95%). The results illustrated that Lachata variety surpass in the characters of number of days to opening flower (59.71 day), number of nodes to first fruiting (3.39 node), boll shedding (2,75%), number of total bolls (32.20 boll/plant), seed index (9.97 g), plant yield (85.29 g) and earliness (77.71%). The interaction between nitrogen fertilization and plant spaces, nitrogen fertilization and varieties, was significant in leaf area and plant yield, while interaction between plant spaces and varieties was significant in plant yield. As for interaction among nitrogen fertilization, plant spaces and varieties was significant in plant yield only. This study concludes that the best combination to give a higher yield of seed cotton is when you use a fertilizer level of 240 kg N/ha, plant space 30 cm with Lachata variety.